Molecular screening of faomt, FAFAD1, and FANAAMT loci in the aroma complex of promising strawberry forms

Abstract

Aroma is an important consumer attribute of strawberry fruits. The main aroma compounds of strawberry fruits include mesifuran, γ-decalactone, and methylanthranilate. Molecular analytical methods can be successfully used to identify promising forms at early stages of ontogenesis. This study presents the results of DNA labeling of FaOMT, FaFAD1, and FanAAMT loci in 16 selected strawberry forms of I. V. Michurin Federal Scientifi c Centre (I.V. Michurin FSC). Identifi cation of target alleles was carried out by DNA analysis using diagnostic DNA markers FaOMT-SI/NO (FaOMT gene), FaFAD1-F/R (FaFAD1 gene), and FanAAMT-F/R (FanAAMT gene). The marker fragment of the functional allele FaOMT+ (mesifuran biosynthesis) was identifi ed in 81.3 % of the forms. In 43.8 % and 37.5 % of the forms, this fragment was found to be homozygous and heterozygous, respectively. The non-functional FaOMT- allele in homozygous state was identifi ed in 18.7% of hybrids. The FaFAD1 gene (γ-decalactone biosynthesis) was identifi ed in 68.7 % of the forms, while the FanAAMT gene (methylanthranilate biosynthesis) was found in 6.2 % of the forms. In 56.2 % of the analyzed genotypes, two genes of the fruit aroma complex are present. The combination of FaOMT and FaFAD1 genes was detected in 50.0 %; the combination of FaOMT and FanAAMT genes was found in 6.2 % of seedlings. A combination of the FaOMT gene (homozygous state of functional allele) and the FaFAD1 gene was found in the 2/2–32, 2/2–41 (Faith × Tea), 3/4–7 (Malwina × Tea) forms. A combination of the FaOMT (homozygous state of functional allele) and FanAAMT genes was detected in the 3/4–6 (Malwina × Tea) hybrid. The forms under study are promising genetic sources of aroma complex loci for further breeding