Genetic polymorphism of promising raspberry varieties determined by SSR genotyping

Abstract

   In this research, we investigate the genetic polymorphism of raspberry varieties and a promising raspberry hybrid bred at the Federal Horticultural Center for Breeding, Agrotechnology, and Nursery. The research objects were the following varieties: Penguin, Skromnitsa, Arisha, Komsomolskaya Pravda, Ivan Kupala, Salut, Lavina, Yubileynaya Kulikova, and No. 10-165-11. To differentiate the studied genotypes by the degree of genetic similarity, an SSR marker analysis was performed using the following 15 loci: RhM011, RiM019, RhM003, RiG001, Rubus123a, Rubus285a, Rubus270a, Rubus275a, RhM043, RhM021, RiM017, RiM015, RcFH01, RiAS01, and RiMY01. Across all 15 loci, unique sets of polymorphic alleles were identifi ed, and genetic profi les for each specimen were compiled. The average number of polymorphic DNA fragments per locus was equal to 3.6. The highest number of polymorphic amplicons per locus reached 6. The results of genotyping were used to calculate the coeffi cients of genetic similarity of the studied varieties. The complied coeffi cient matrix was subsequently used for cluster analysis. As a result of clustering, groups of genetically similar varieties were identifi ed. The largest group included Arisha, Komsomolskaya Pravda, Salut, Yubileynaya Kulikova, Penguin, and No. 10-165-11. Lavina, Skromnitsa, and Ivan Kupala specimens showed the largest genetic variations. The data obtained elucidates the current understanding of the phenotypic features of raspberry varieties and can be used when developing eff ective crossing schemes in raspberry breeding.