Prevalence of viral diseases and isolation of candidates in nuclear stock plants of raspberry ex situ

On raspberries, currently about 30 viral diseases are known in the world that can reduce the yield and its quality. According to the results of previous studies in the Moscow region, the prevalence of viruses on raspberries was: Arabis mosaic virus(ArMV) – 14 %, Raspberry ringspot virus(RpRSV) – 30 %, Strawberry latent ringspot virus(SLRSV) – 16 %, Tomato black ring virus (TBRV ) – 18 %, Raspberry bushy dwarf virus (RBDV) – 39 %. Viruses spread in agrocenosis with infected planting material, with tools, with pollen and seeds, nematodes – longidorids (Xiphinema diversicaudatum – ArMV and SLRSVvector, Longidorus elongatus – RpRSV and TBRVvector). The harmfulness of viruses on raspberry plants consisted inreducingthe productivity by 21 %, fruit masse– by 26 % compared with virus-free plants. The aim of the study was to study the species composition of viruses on raspberries to identify candidates for the nuclear stock plants. In serological tests, the ELISA sandwich version was used according, for analysis, diagnostic kits from Loewe (Germany) were used. Leaves were taken as samples. The results of analyzes were recorded on a Stat Fax 2100 hotometer at a wavelength of 405 and 630 nm. RBDV virus RNA was isolated using the CytoSorb kit, followed by RT-PCR. The species composition of viruses on raspberry varieties was studied under ex situ conditions. The total prevalence of viruses was 29 % with the predominance of the RBDV virus (19 %). 102 candidates for nuclearstockplants of 22 varieties of raspberries were identified. After confirming the virus-free status of raspberry plants by PCR, they will receive the category “nuclearstock plant”.

1. Евдокименко С.Н., Упадышев М.Т., Якуб И.А., Метлицкая К.В. Кустистая карликовость малины: проблемы и пути решения // Плодоводство и ягодоводство России, 2013.– Т. ХХХVI, ч. 1.– С.167–174.

2. ConverseR. H., Ellis M. A., Converse R. H., Williams R. N. and B. Williamson Diseases caused by virus esand virus-likeagents. In: CompendiumofRaspberryand Blackberry Diseases and Insects. American Phytopathological Society, St. Paul, MN, USA, 1991.

3. Упадышев М.Т., Метлицкая К.В., Петрова А.Д., Донецких В.И. Распространенность, вредоносностьвирусныхболезней малины и современные методы оздоровления // Сборник науч. трудов «Современные тенденции устойчивого развития ягодоводства России» (земляника, малина).– Воронеж: Кварта, 2019. – Т. 2.– С. 292-307.

4. Martin R.R., MacFarlane S., Sabanadzovic S., Quito D., Poudel B., Tzanetakis I.E. Virus esandvirus diseases of Rubus // PlantDis., 2013. – 97(2). – Р. 168‐182. doi:10.1094/PDIS-04-12-0362-FE

5. Упадышев М. Т., Метлицкая К. В., ПетроваА. Д., Тихонова К.О. Закономерности распространения вредоносных вирусов в агроценозах малины и земляники садовой // Плодоводство и ягодоводство России, 2015.– Т. 41.– С. 366-371.

6. Pupola N., Lepse L., Kale A., Morocko-Bicevska I. Occurrence of RBDV in Latvia and virus elimination in vitro by chemotherapy // Scientific works of the Lithuanian Institute of Horticulture and Lithuanian University of Agriculture. Sodininkysteir Darzininkyste, 2009.– 28 (3).– Р. 165-172.

7. Упадышев М.Т., Тихонова К.О., Метлицкая К.В. Вредоносность вирусов на малине в полевых условиях // Плодоводство и ягодоводство России,2016. – Т. 45.– С. 188-192.

8. Лукьянова Е.А. Вирусные болезни ягодных растений в ЦЧР. – Мичуринск:МГПИ, 2007. – 115 с.

9. Куликов И. М., Завражнов А. И., Упадышев М. Т., Борисова А. А., Тумаева Т.А. Научно-методические основы индустриальной агротехнологии производства сертифицированного посадочного материала плодовых и ягодных культур в Российской Федерации // Садоводство и виноградарство, 2018. – №1. – С. 30-35.

10. Упадышев М.Т., Мельникова Н.Н., Петрова А.Д., Суркова О.Ю., Метлицкая К.В., Походенко П.А., Саунина И.И. Диагностика вирусов семечковых и косточковых культур методами ИФА и ПЦР. – М.: ВСТИСП, 2008.– 35 с.