EFFICIENCY EVALUATION OF COMMERCIAL KITS FOR DNA ISOLATION FROM LAVANDINE (LAVANDULA × INTERMEDIA EMERIC EX LOISEL.)
https://doi.org/10.31676/2073-4948-2019-59-286-293
Abstract
The paper describes the results of the genomic DNA isolation from the Rabat variety lavandin leaves using ready-made commercial kits. The data on DNA concentration and its quality determined spectrophotometrically and by using agarose gel electrophoresis аnd automated electrophoresis system Agilent 4200 TapeStation are presented. Conclusions of suitable NucleoSpin® Рlant II Midi kit for DNA isolation from lavandin leaves are made.
About the Authors
I. V. Bulavin
Nikitsky Botanical Garden — National Scientific Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
V. A. Brailko
Nikitsky Botanical Garden — National Scientific Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
O. A. Grebennikova
Nikitsky Botanical Garden — National Scientific Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
M. S. Andreev
Nikitsky Botanical Garden — National Scientific Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
O. V. Krivenko
Nikitsky Botanical Garden — National Scientific Center of the Russian Academy of Sciences;
A. O. Kovalevsky Institute of Biology of the South Seas of the Russian Academy of Sciences
Russian Federation
Russian Federation
I. V. Mitrofanova
Nikitsky Botanical Garden — National Scientific Center of the Russian Academy of Sciences
Russian Federation
Russian Federation
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Review
For citations:
Bulavin I.V., Brailko V.A., Grebennikova O.A., Andreev M.S., Krivenko O.V., Mitrofanova I.V. EFFICIENCY EVALUATION OF COMMERCIAL KITS FOR DNA ISOLATION FROM LAVANDINE (LAVANDULA × INTERMEDIA EMERIC EX LOISEL.). Pomiculture and small fruits culture in Russia. 2019;59:286-293. (In Russ.) https://doi.org/10.31676/2073-4948-2019-59-286-293
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