OBTAINING OF STERILE CULTURE FROM SEEDS AND CLONAL MICROPROPAGATION OF FICUS CARICA L
Abstract
Technology of Ficus carica L. clonal micropropagation is worked out. The expedience of alterating of nutritive medium with TDZ and 2-iP during microcutting is noticed. The induction of development of all axillar buds of explant occurs on plant medium with 0,2 mg/l TDZ. Microshoots without any features of vitrifi cation develop on plant medium with 1 mg/l 2-iP. The obtained results can be used to develop a strategy for preserving the gene pool of figs in the collection in vitro.
About the Authors
A. A. Krinitsina
Lomonosov’s Moscow State University
Russian Federation
Russian Federation
O. A. Churikova
Lomonosov’s Moscow State University
Russian Federation
Russian Federation
References
1. Шишкина Е. Л. Создание генофонда инжира в Никитском ботаническом саду и его использование // Тр. Никитского ботанического сада, 2010. - Т. 1. -№ 32. - C. 184-188.
2. Zohary D., Hopf M. Domestication of Plants in the Old World // Oxford University Press, 1988. - Oxford, UK. - 328 p.
3. Рихтер А. А., Виноградов Б. А., Марчук Н. Ю., Шишкина Е. Л. Аромат соплодий инжира Ficus carica L. // Інтродукція рослин, 2014. - № 3. - С. 85-91.
4. Ядров А. А., Синько Л. Т., Казас А. Н., Шолохова В. А. Орехоплодные и субтропические плодовые культуры - Симферополь: Таврия, 1990. - 160 с.
5. Podgornik M., Vuk I., Vrhovnik I., Mavsar D.B. A survey and morphological evaluation of fig (Ficus carica L.) genetic resources from Slovenia // Scientia Horticulturae, 2010. - V. 125. - P. 380-389.
6. Sadder M. T., Ateyyeh A. F. Molecular assessment of polymorphism among local Jordanian genotypes of the common fig (Ficus carica L.) // Scientia Horticulturae, 2006. - V. 107. - Р. 347-351.
7. Pasqual M., Ferreira E. A. Micropropagation of fig tree (Ficus carica L.) / Jain S. M., Häggman H. (eds.) // Protocols for micropropagation of woody trees and fruits, 2007. - P. 409-416.
8. Taha R. A., Mustafa N. S., Hassan S. A. Protocol for micropropagation of two Ficus carica cultivars // World Journal of Agricultural Sciences, 2013. - V. 9. - No 5. - P. 383-388.
9. Fraguas C. B., Pasqual M., Duta L. F., Cazetta J. O. Micropropagation of fi g (Ficus carica L.) ‘Roxo de Valinhos’ plants // In Vitro Cellular and Developmental Biology - Plant, 2004. -V. 40. - No 5. - P. 471-474.
10. Murashige T., Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue culture // Physiol. Plant., 1962. - V. 104. - P. 473-497.
Review
For citations:
Krinitsina A.A., Churikova O.A. OBTAINING OF STERILE CULTURE FROM SEEDS AND CLONAL MICROPROPAGATION OF FICUS CARICA L. Pomiculture and small fruits culture in Russia. 2017;49:178-181. (In Russ.)
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