MOLECULAR METHODS IN THE DIAGNOSTICS OF APPLE TREE PATHOGENS IN BELARUS
https://doi.org/10.31676/2073-4948-2019-56-118-123
Abstract
In the article the results of molecular-genetic identifi cation of the pathogens causing brown rot, anthracnose and quarantine disease - fi re blight are presented. Molecular analysis data of rDNA Venturia inaequalis isolates - causative agent of apple scab are presented. Genetic heterogeneity of pathogen populations has been revealed.
About the Authors
E. V. Vasekha
Institute of Plant Protection
Russian Federation
Russian Federation
V. S. Komardina
Institute of Plant Protection
Russian Federation
Russian Federation
O. V. Dichkovskaya
Institute of Plant Protection
Russian Federation
Russian Federation
References
1. Высокопроизводительные и высокоточные технологии и методы фитосанитарного мониторинга / Под ред. И. В. Гричанова. - СПб: ВИЗР, 2009. - 84 с.
2. Schnabel G., Schnabel E. L., Jones A. L. Characterization of ribosomal DNA from Venturia inaequalis and its plylogenetic relationship to rDNA from other tree-fruit Venturia species // Phytopathology, 1999. - Vol. 89, №. 1. - P. 100-108.
3. Кулик Е. В., Комардина В. С., Саросек А. И., Евтушенков А. Н. Молекулярная дифференциация изолятов Venturia inaequalis // Весці Нацыянальнай акадэміі навук Беларусі, 2013. - № 3. - С. 36-40.
4. Côté M.-J., Tardif M.-C., Meldrum A. J. Identifi cation of Monilinia fructigena, M. fructicola, M. laxa, and Monilia polystroma on Inoculated and Naturally Infected Fruit Using Multiplex PCR // Plant Disease, 2004. - Vol. 88, № 11. - P. 1219-1225.
5. Hughes K. J. D., Fulton C. E., McReynolds D., Lane C. R. Development of new PCR primers for identifi cation of Monilinia species // Bulletin OEPP/EPPO Bulletin, 2000. - № 30. - P. 507-511.
6. Gariépy T. D., Lévesque C. A., Jong S. N., Rahe J. E. Species specifi c identifi cation of the Neofabraea pathogen complex associated with pome fruits using PCR and multiplex DNA amplifi cation // Mycol. Res., 2003. - Vol. 107, № 5. - P. 528-536.
7. Peśicová K., Kolařik M., Hortová B., Novotný D. Diversity and identifi cation of Neofabraea species causing bull’s eye rot in Czech Republic // Eur. J. Plant Pathol., 2017. - Vol. 147. - P. 683-693.
8. Lagonenko A. L., Komardina V. S., Nikolaichik Y. A., Evtushenkov A. N. First report of Erwinia amylovora fi re blight in Belarus // J. Phytopathology, 2008. - Vol. 156. - Р. 638-640.
9. Lagonenko A. L., Lagonenko V. Y., Nikolaichik Y. A., Evtushenkov A. N. Detection of Erwinia amylovora by PCR with primers to the hrpN gene // Acta Hort., 2011. - Vol. 896. - P. 85-91 (Abstract).
Review
For citations:
Vasekha E.V., Komardina V.S., Dichkovskaya O.V. MOLECULAR METHODS IN THE DIAGNOSTICS OF APPLE TREE PATHOGENS IN BELARUS. Pomiculture and small fruits culture in Russia. 2019;56:118-123. (In Russ.) https://doi.org/10.31676/2073-4948-2019-56-118-123
Views:
401
Email this article 
