DETERMINATION OF SENSITIVITY OF PROBES FOR THE DIAGNOSIS OF PHYTOPHTHORA BLIGHT OF RASPBERRY AND STRAWBERRY IN THE SETTING OF PCR “IN REAL TIME”
Abstract
As a result of the research, a technique for multiplex PCR “real-time” was elaborated to identify the main pathogens of phytophthora root rots of raspberry and strawberry, including quarantine biotype Ph. fragariae. The selected probes allowed to reliably identify the causative agents of late blight root rot of raspberry and strawberry in a concentration from 2.0x10-5 mg/ml to 3.3x10-7 mg/ml.
About the Authors
S. E. Golovin
FSBSI ARHIBAN
Russian Federation
Russian Federation
M. B. Kopina
All-Russian Plant Quarantine Center
Russian Federation
Russian Federation
References
1. Головин С. Е. Проблемы распознавания фитофторовых грибов на ягодных, декоративных и цветочных культурах и разработка способов их диагностики // Плодоводство и ягодоводство России, 1999. - Т. 6. - С. 197-204.
2. Duncan J. M., Kennedy D. M., Seemuller E. Identities and pathogenicities of Phytophthora sp. causing root rot of red raspberry // Plant Pathology, 1987. - V. 36. - № 7. - P. 276-289.
3. Li M., Inada M., Watanabe H., Suga H., Kageyama K. Simulataneous Detection and Quantification of Phytophthora nicotianae and P. cactorum and Distribution Analyses in Strawberry Greenhouse by Duplex Real-time PCR // Microbes and Enviriments, 2013. - Vol. 28. - N.2. - P. 195-203.
4. Копина М. Б. Фитофторозные корневые гнили малины и земляники, методы их диагностики: Автореф. дисс.. к. с.-х. наук. - М., 2013. - 23 с.
5. Головин С. Е., Копина М. Б. Фитофторозные корневые и прикорневые гнили малины и земляники, современные методы их диагностики (монография). - М.: ГНУ ВСТИСП, 2014. - 196 с.
6. Doyle J. J., Doyle J. L. Isolation of plant DNA from fresh tissue // Focus. Life technolog. inc., 1990. - Vol. 12. - P. 13-15.
7. Schena L., Duncan J. M., Cooke D. E. L. Development and application of a PCR-based molecular tool box for the identification of Phytophthora species damaging forests and natural ecosystem // Plant Path., 2008. - V. 57. - № 7. - P. 64-75.
8. Bonants P. J. M., van Gent-Pelzer М., Hooft man R., Cooke D. E. L., Guy D. C., Duncan J. M. A combination of baiting and different PCR formats, including measurement of real-time quantitative fl uorescence, for the detection of Phytophthora fragariae in strawberry plants // European Journal of Plant Pathology, 2004. - V. 110. - Р. 689-702.
9. Duncan J. M., Kennedy D. M. Effect of temperature and host genotype on the production of inoculum by Phytophthora fragariae var. fragariae from the roots of infected strawberry plants // Plant Pathology, 1995. - V. 44. - № 1. - P. 10-21.
Review
For citations:
Golovin S.E., Kopina M.B. DETERMINATION OF SENSITIVITY OF PROBES FOR THE DIAGNOSIS OF PHYTOPHTHORA BLIGHT OF RASPBERRY AND STRAWBERRY IN THE SETTING OF PCR “IN REAL TIME”. Pomiculture and small fruits culture in Russia. 2018;55:246-249. (In Russ.)
Views:
228
Email this article 
