On the basis of integrated assessment, sweet cherry varieties Yantarnaya Savel’yeva, Mulatka and Lika, characterized by relative winter resistance, complex resistance to moniliosis and coccomicosis, were revealed. The given varieties are promising for commercial use in the southern part of the Central chernozem region. The degree of freezing of wood and vegetative buds after artificial freezing at –35 °C (II component) did not exceed 2.3 points and 1.6 points, respectively. In the field conditions, the degree of damage of these varieties to monilial burn was no more than 0.3 points, to coccomycosis it was 0.7 points, to monilial fruit rot it was 1.3 points. The yielding capacity of these varieties varied from 6.01 to 7.12 t/ha depending on the variety, which amounted to 123.7-146.5 % of the control. Variety were characterized by high commodity-consumer qualities of fruits. The average fruit weight was 5.1-7.1 g, and the overall taste evaluation was 4.6-4.8 points.

This article highlights the results of research on the resistance to powdery mildew (Sphaerotheca mors-uvae (Schw.) Berc. Etcurt.) of black currant (Ribes nigrum L.) and red currant (Ribes rubrum L.) varieties of Russian Research Institute of Fruit Crop Breeding. The research was conducted on the natural infectious background of collection sites and areas of primary variety study of black currant planted in 2014, with plant spacing 3×1 m, and red currant planted in 2015, with plant spacing 3.5×0.5 m. As objects of the study, 29 black currant varieties and 17 red currant varieties were taken. The purpose of the research was to assess the resistance to powdery mildew of black currant and red currant varieties and to reveal highly resistant varieties based on screening for further use in breeding and production. In the breeding of black currant for immunity to powdery mildew, the most effective areas were those where donors of four resistance oligogens were involved, of which the derivatives of nutmeg currant Ribes glutinosum (Sph3 gene) and the Sunderbyn — II variety (R gene) were most widely involved in the crossings. In red currant breeding, the derivatives obtained from intervarietal crosses of different genetic origin were used as sources of resistance to powdery mildew. Highly resistant varieties of black currant were identified on the basis of screening: Arapka, Ariadna, Assol, Gamma, Gratsia, Iskushenie, Kipiana, Kreolka, Narianna, Nyura, Oasis, Ocharovanie and Podarok Leta. In the main mass of varieties the degree of damage varied from 1.5 to 2 points: Azhurnaya, Ladushka, Monisto, Blagosloveniye, Blakeston, Orlovia, Orlovsky Vals, Orlovskaya Serenada, Dachnitza, Desertnaya Ogoltzovoy, Zagliadenie, Chernaya Vual, Zusha, Ekzotica, Asya, Valentinovka, Gazel and Niva. Blakeston was previously considered as highly resistant in the field, but in 2019, with a strong infectious load, the lesion score was 1.5. All these varieties may be recommended for commercial growing of black currants. The use of highly resistant currant varieties in commercial cultivation will reduce the pesticide load on agrocoenosis, economic costs and make it possible to obtain environmentally friendly products.

The article considers the results of experiments on the study and selection of sources of valuable features from the world collection. The aim of the research is to create new varieties of winter triticale with high features of productivity (over 12 t/ha) and grain quality in comparison with the Victor and Hermes standards. Special attention in the experiments is paid to the search for genotypes that are promising for use in breeding programs for different types of crossing, obtaining hybrid populations, identifying forms, lines with a complex of economically useful features. Breeders of Federal Research Center “Nemchinovka” for a number of years received varieties of winter triticale, competitive in Russia and abroad, able to form a crop of more than 10 t\ha of quality grain. However, their disadvantages are tallness, susceptibility to dangerous diseases (snow mold, septoriosis), and significant variability in grain harvest over the years (2.9). The authors have identified sources of economic and valuable features from the collection of N. I. Vavilov All-Russian Institute of Plant Genetic Resources. This includes the winter-hardy variety Tsekad 90, drought-resistant Doctrina 110, short-stemmed Legion, Kentavr, yielding large-grain Efremovskaya, etc., successfully used in breeding. Highly productive lines have been created (No.6 408-19-71, No.618-176, No.878-1-25, No.690-1-19) and varieties (Gera, Kapella, Arktur) with a fixed grain harvest of more than 10t\ ha, adaptive to the natural conditions of the non-Chernozem zone and the middle Volga region.

The article presents the results of research on the variety study of black currant plants (Ribes nigrum L.) obtained using the method of clonal micro-propagation in vitro. As objects, regenerated plants of varieties of breeding of the Bashkir Research Institute of Agriculture were involved: Karaidel and Chishma, as well as the Seyanets Golubki variety. The research was conducted at the Kushnarenkov о breeding center for fruit and berry crops and grapes in the period from 2017 to 2019. Phenological observations, accounting of winter hardiness and drought resistance, general condition of plants, accounting of the degree of plant damage by diseases and pests, and the determination of the dormancy period were carried out.

The aim of the conducted research was to study the dynamics of nitrogen content in various organs of apple trees (spurs, annual shoots, branch wood) in the fruit-bearing orchard in different periods of the growing season. Nitrogen content was monitored at the end of the growing season (November), during the beginning of the growing season (April) and during the period of active fruit growth (July). The research was carried out in an intensive apple orchard in the Tambov region on the next varieties: ‘Antonovka Obyknivennaya’, ‘Zhigulevskoye’, ‘Orlik’, ‘Spartan’, ‘Bogatyr’, grafted on rootstock 62-396. The nitrogen content was determined in dried and crushed plant material according to the Kjeldahl method. As a result of research, it was found that the high content of nitrogen in the wood of apple trees was recorded in the autumn period, in the future the level of nitrogen decreased and reached the minimum value by the spring. In summertime, the content of nitrogen in 2-4 and 5-6 years old wood of all studied varieties increased in comparison to autumn and spring. The largest content of nitrogen was found in wood of ‘Zhigulevskoye’ variety was 0,82 % (2-4 years old wood) and 0,86 % (5-6 years old). This is more than in autumn on 0,30 % and 0,24 %, and in spring on 0,39 % and 0,35 % respectively. Based on the data obtained, the content of this nutrient in the apple tree wood in the autumn could be corrected by the application of nitrogen fertilizers during growing season, which directly affect the accumulation of reserve substances and tissue aging. It makes a positive impact on the development of the future crop and the preparation of trees for the winter.

The article presents experimental data on the electrical conductivity of tissues of grafted apple and pear crops to identify patterns of electrical conductivity in qualitative accretion of grafting components at early stages. For the fastest implementation of the varieties and clonal rootstocks obtained by breeding, it is important to select stable scion-stock combinations. The main task remains to detect incompatibility of the components of grafts at an early age, since its manifestation in adult plants causes significant damage to production. The studies were carried out in different years on winter and summer grafts. Patterns of the electrical conductivity dynamics are established: with qualitative fusion of grafting components, a monotonous increase in electrical conductivity is observed when the values of electrical conductivity change between the 1st and 14th day within 80 μS. In case of poor quality growth of grafting components, values of electric conductivity of tissues practically do not change (±5-10 μS). In rotting tissue (in visually unchanged tissues) values of electrical conductivity of all components of grafting in the period from the 8th to the 18th day after grafting are sharply increased by 100 mS, which is connected with water ion exchange disorder in tissues. When reproduction by budding in its initial period, in compatible combinations, the drop in tissue conductivity values at the grafting site was no more than 20 μS compared to the indications on the rootstock above the grafting site. On the 16th day of measurements, the readings of the electrical conductivity tissues at the grafting site and on the reverse side were practically unchanged (± 5 μS) — this indicate a gradual repair of the grafting tissues.

Introduced grape varieties were studied in the south of Western Siberia in order to identify the effects of unfavourable factors of the growing season on the ripening of grape shoots. Weather conditions in the south of Western Siberia during the vegetation periods 2012-2017 were characterized by large fluctuations in water and temperature conditions; there were sharp changes not only in different years, but also during one season. Compared with long-term average data, a significant increase of relative air humidity and the amount of precipitation was noted from May to September. In 2016-2017 sharp changes of the amount of precipitation by months were marked (dry June, over-moistened July). The most unfavorable weather conditions for grape crop were the conditions of the growing season of 2013 with excessive rainfall (410.3 mm), very low average monthly air temperatures in May, June, July, September and the sum of active temperatures (2031.7°C). Under the influence of meteorological conditions, the terms of the beginning of the studied phenological phase changed very significantly over the years (30-62 days). Shoots ripening in years with favourable weather conditions (2012, 2015) began from July 14-August 1, with unfavorable (2013, 2017) — September 11-15. In one grape variety, insignificant variability of the degree of the studied character was revealed by years, in two — medium, in eight — high. Shoots ripened better in warmer and less humid 2012 (66.8-94.1 %) and in 2015 (58.4-92.5 %). Varieties with very poor ripening (33.0-46.9 %) Arkady, Kodryanka, Tomaysky are unsuitable for cultivation under production conditions and recommended for cultivation in amateur gardens with additional shelter. Varieties with maximum ripening of shoots on average over the years were selected from the American group White Pearls, Latvian Pink (83.1-84.9 %), Amurian Adel, Vaskovsky Souvenir, Hasan Bousa (65.5-79.3 %).

The research was carried out on the basis of the laboratory of stability of physiology of fruit plants at All-Russian research Institute of Breeding of Fruit Crops in 2017-2018. The object of research was the scion/stock combinations of Novella and Turgenevka cherries/82987, C-2-180, 74332, 74340, 74336, 74324 breeding of the Institute. In the study of winter hardiness of cherry, special attention was paid to the ability of varieties to withstand low temperatures in mid-winter. The aim of the research was to study the maximum frost new scion/stock combinations of cherries by artificial freezing and selection of the hardiest of them. The results of the research noted that in the recovery period of organic dormancy in late December, when the cherry plants acquire the maximum hardened state, study scion/stock combinations were characterized by high resistance to frost of bark and wood of annual branches. They noted frost resistance of vegetative buds with reversible damage. Significant differences in the freezing vegetative buds, bark and wood between scion/stock combinations of sweet cherries are shown. Novella and Turgenevka cherry varieties on clonal rootstocks 74332, 74336, characterized by the greatest potential frost resistance of vegetative buds and tissues of annual branches at lower t= -35°C (winter hardiness component II) at the end of December. It is shown that all cherry scion/stock combinations have a low potential frost resistance of generative buds. After exposure to t= -35°C, living rudiments of flower buds preserved only in cherry scion/stock combinations Novella/74332, Turgenevka/74340, 74336 (from 4.0 to 15.4 %). According to the results of artificial freezing scion/stock combination Novella/74332 and Turgenevka / 74332, 74336 were revealed, which were characterized by the maximum frost resistance of generative, vegetative buds, bark and wood.

Different level of reaction of apple varieties immune to scab breeding of I.V. Michurin Federal Scientific Center on the effect of withering and high temperatures (heat shock) was revealed. The degree of heat resistance and drought resistance of plants was determined. Varieties with high water holding capacity and restoration of hydration and which merit be use in breeding as genetic sources of these characters have been identified.

Recently, urban farms for growing green and berry crops with artificial light are gaining popularity. The effect of different parts of the spectrum in the visible region on vegetable and berry crops has been studied extensively, but the influence of ultraviolet and far red radiation requires more detailed studies. The purpose of the work was to study the influence of the spectral composition of the radiation of LEDs on the flowering and fruiting of strawberries. For research Elan F1 and Florian F1 hybrids of everbearing strawberries were chosen. Plants were grown in phytocameras on racks with artificial LED lighting. It has been established that with a fraction of the far red component of at least 15 % in radiation, an increase in leaf area and the length of peduncles occurs.

Using the method of artificial freezing, the analysis of frost resistance of annual shoots of varieties, forms and species of ornamental apple trees in the middle of winter was carried out. The purpose of work was to evaluate the genotypes of apple trees in the ornamental genetic collection of the Michurin Federal Scientific Center on maximum frost resistance. Freezing was carried out at a temperature of –40°C after standard hardening –5°C and –10°C (each stage for five days). The rate of temperature decrease was 5°C per hour. After reaching the required temperature, the shoots were frozen for 18 hours. Genotypes with maximum resistance to temperature drop to -40 °C were identified. This is the variety of Jay Darling, who is not marked by defeats of the wood. The Royalty variety is characterized by resistance to low temperatures at the control level Antonovka Оbyknovennaya. The academician of the Russian Academy of Sciences N. I. Savelev obtained promising genotypes of ornamental apple trees using the frost-resistant Yakutskaya I form and columnar seedlings with high aesthetic value. A high degree of resistance was found in columnar ornamental forms 7 II-69 and 7 II-67 — wood damage did not exceed 0.4 points. Relatively high frost resistance with a degree of wood damage from 1.1 to 2.0 points was recorded in the species M. transitoria, selected seedlings 7 I-103, 7 II-68, 7 II-72, 7 II-58, 7 I-31 and in variety Professor Sprenger. The degree of freezing of the buds in all studied genotypes did not exceed 1.0 points, which indicates that the temperature -40 о С in the middle of winter was not critical for them. The selected genotypes can be successfully used in gardening and used in further breeding.

The article presents the results of the collection study of new varieties of strawberry breeding in All-Russian Horticultural Institute for Breeding, Agrotechnology and Nursery in the conditions of the Ural region (on the example of the Orenburg region). Unfavorable climate features for strawberries in the Orenburg Urals are low negative temperatures at the beginning of winter in the absence of snow cover and dry conditions during the growing season. According to the results of the study, Bereginya, Tsaritsa, Kokinskaya, Rosinka, Studencheskaya varieties were distinguished by good adaptation, high productivity (more than 0.2-0.3 kg/bush), and large — fruiting (average weight-more than 9.0 g). According to the indicated parameters, these varieties surpassed the zoned variety Orlets.

The work is devoted to the study of peculiarities of the hereditary potential of soft winter wheat productivity in the conditions of the Belgorod region. The study of yield of winter wheat varieties was conducted for two years. In 2016, 8 varieties zoned in our (fifth) region of this culture of breeding of the Belgorod and Kursk regions and 8 of other origin were studied. It is established that the yield in the first group of varieties made up of 5.31 t/ha, and in the second group it is 3.76 t/ha [(the Least Significant Difference) LSD_0.95 = 0.73 t/ha]. In 2017 continued the test. The second group, of southern origin, included 15 varieties. The following results were obtained: the first group had a yield of 6.45 ±0.24 t/ha and the second of 4.64 ±0.68 t/ha (t = 6.08***, p < 0.001). In the second group were the varieties approved for use in our region (4 varieties from 15) and rated their productivity. Their productivity made up 4.92 ±0.41 t/ha. The difference from the first group was 1.53 t/ha, which was statistically significant (t = 3.21*, p < 0.05). The obtained data confirm the results of varietal groups estimations yield for previous years (2008-2010). In this case, the group of Krasnodar-Zernograd varieties (4) breeding had an average yield of 3.25 t/ha for three years, 3.00 t/ha was of the Moscow origin (2), and varieties created in the Belgorod area (5) were un yield of 4.39 t/ha. Presented data indicate the need to expand breeding work in regions of the Russian Federation, which will allow more efficiently use of the potential in winter wheat culture. The positive temperature dynamics in recent years has not increased the adaptability of varieties of southern origin to the conditions of the Belgorod region.

In the conditions of the South Urals, an economic and biological assessment of 9 plum varieties introduced from the Altai Mountains (the village of Chemal) was carried out in order to assess and identify the best ones for breeding and practical use. According to the research results, accessions with a complex of economically valuable traits were identified. Accessions G 7-126 (26.1 kg per tree) and G 9-150 (23.2 kg per tree) were the most productive in the years of research. Freezing of generative buds in the winter of 2017/2018 was observed in the accessions G 9-150, G 7-126 and amounted to 0.5 points (not more than 10 % of the buds), in the remaining variety specimens from 2.5 to 3.0 points ( buds death was 25-50 %), which led to yield reduction. Late fruit ripening period (second decade of September) was noted for the accessions G 7-126 and G 9-150, the remaining the accessions (77.8 %) have a medium late ripening period. The accession G 9-150 was distinguished for good winter hardiness, weakness, productivity, late ripening, quality and taste of fruits. Three accessions are recommended for breeding — G 12-132, G 12-150 (winter hardiness, weakness, fruit quality), G 7-126 (accession with anthocyanin color of the pulp of the fruit).

The results of the study of black currant varieties in the Botanical garden of the Institute of biology of the Komi Scientific Center of the Ural branch of the Russian Academy of Sciences are presented. The object of study was to determine the degree of longevity and select promising varieties in the climatic conditions of the middle subzone of the taiga. The following economically useful characteristics were analyzed: winter hardiness, phenological phases, productivity, largefruiting (berry weight). Methods of grouping and evaluating differences among groups were used. It is established that the varieties of black currants are winter hardy and remain productive in the conditions of middle taiga subzone for ten years or more. The varieties ‘Cerera’ (2.9 kg per bush), ‘Naslednica’ (2.6) and ‘Lentyaj’ (2.4) are found to be the most productive. Large-fruited varieties are ‘Seyanec Golubki’(1.58 grams), ‘Naslednica’ (1.54) and ‘Lentyaj’ (1.5). The prospects of growing black currant in the middle subzone of the taiga are confirmed. The promising varieties are revealed: ‘Naslednica’, ‘Lentyaj’ and ‘Cerera’.

The paper provides an assessment of the frequency of apple fruiting of different maturation periods growing on the lower terrace of the Vs. M. Krutovsky Botanical garden in the period from 2015 to 2018 according to the following parameters: the number of fruit-bearing trees, the number of fruits on the tree, yield. In the conditions of the Botanical garden, located in the green zone of Krasnoyarsk, the timing of the onset of removable fruit maturity varies significantly. The variability of the fruit formation period depends on the weather conditions in the summer and primarily on the temperature regime. The optimal time for collecting varieties of the summer ripening period is August, and for winter varieties is September, since by this time the fruits of these groups acquire a characteristic color and size. It was found that during the research period, most of the trees of the presented varieties fruited annually. Varieties and individual specimens with an annual plentiful fruiting and maximum fruit weight of fruits are selected: Bismark, Belyy Naliv, General Orlov, Grushovka moskovskaya, Zolotoy Ship, Papirovka, which is a positive factor in a sharply continental climate. It was found that in Siberia, varieties adapted to the European zone have successfully adapted and can be grown in these and similar climatic conditions and can be recommended for further breeding.

Emistim is one of the next-generation growth regulators that are active in plant organisms in very low concentrations. The purpose of this work was to study the effect of plant growth regulator Emistim on the growth and development of representatives of the genus Hosta Tratt., Iris L., Narcissus L. based at the South-Ural Botanical Garden-Institute of Ufa Federal Research Center of Russian Academy of Sciences. The research method is a field experiment. The scheme of the experiment included the following options: control is plants without treatment; experience is spraying of vegetative parts of plants with Emistim. Seed production was estimated by the method of I. V. Vainagy. As a result of the experiments, a significant change in the biometric parameters of the studied plants was shown. The Hosta increased the following indicators: plant height (up to 46 %), petiole length (up to 30 %), the number of flowers on one peduncle (up to 56 %), the number of fruits on one peduncle (up to 109 %), potential seed productivity (up to 51 %), etc. Irises have also been positively affected: the size of the length of the leaf of the shortened shoot changed (up to 32 %), the width of the peduncle sheet (up to 20 %), the width of the standard (up to 27 %). Narcissus showed an increase in the diameter of the flower (up to 25 %). The result of the influence of the plant growth regulator Emistim significantly depends on the species and varietal characteristics of the studied genera. Each object requires the selection of individual physiologically active substances and optimal conditions for their use. However, it should be noted that the use of this preparation on the studied crops is a promising direction for the practice of crop production.

Long-term results of the variety study of black currant collection in the Middle Urals are presented. The purpose of the work was to study the collection fund of black currant on economically useful features. The peculiarities of weather conditions during the research period 2012-2018 are described, and phenological observations are presented. Based on the results of four years of study, varieties with high productivity, large-fruited, with high taste qualities of berries, resistant to Septoria and Bud mite were revealed. Varieties with economically useful features were identified: Spas for high productivity and large-fruiting, very late maturation; Voevoda, Kazkova, Kozatska, Podarok Ilinoy, Sofievska, Udalec, Shaman — for high and stable yield, high commodity qualities of berries; Bariton, Ben Alder, Udalets, Shaman - for resistance to Bud mite.

In I. V. Michurin Federal Scientific Center a unique genetic collection of pears, numbering more than 300 genotypes was created. As a result of the study of the peculiarities of growth and development of various pear varieties the influence of the growth force, crown diameter and other parameters on yield has been revealed. Based on the conducted research, forms with restrained growth were identified: Pamayty Yakovleva, Augustovskaya Rosa, Smuglyanka, Hera, Allegro, Pervomaiskaya.

The article presents a 3D model of an automated manipulator with a computer vision system for harvesting horticultural crops. It was elaborated an algorithm of the computer vision system to determine the boundaries of the berries and theirdegree of ripeness. The results of field experiments, measuring the area of berries at different times of the day are given. It has been established that thelighting conditionshave a significant impact under on the detection of borders and areas of strawberries, and their degree of ripeness.

Data were obtained on the basis of the results of research carried out on the territory of the educational and experimental farm of the Timiryazev State Agrarian University, in Moscow during 2018-2019. As a result of the surveys, the most dangerous diseases and pests of pome crops on the territory of this farm were established. The most resistant apple and pear varieties to major diseases have been identified. Peculiarities of development of alternariosis on pear are described, the harmfulness of the disease on pear and apple seedlings is noted. A possible role in the transfer of alternariosis infection from garden-protective plantations and weed vegetation to fruit trees was noted. A possible role has been established in the transport of septoriosis, powdery dew infection from dicotyledonous weeds plants. The peculiarities of the spread of infection under the influence of wind direction are noted. The results and peculiarities of the application of various methods of scaring birds in the orchard are presented. As a result of route surveys the most harmful weed plants have been identified. The possibility of using herbicides of different mechanism of action in fruit gardens for weed control has been studied. High efficiency and relative safety of application of herbicides of contact action in nursery fields, operational orchards and for control of piglets on fruit trees are shown. Recommendations are given for the use of soil and systemic herbicides of soil in seedlings beds, the first and second fields of the nursery, as well as in the process of production of large-scale planting material and operational orchards of fruit crops. The safety of the herbicides in question is established when used in accordance with the recommended methods of use.

In the production of planting material, it is important to choose a healthy, highly productive source material, with typical varietal characteristics, and, if necessary, their disinfection), as well as reliable protection against dangerous pests at further stages of cultivation. This requires a scientifically based methodological approach and phytosanitary measures in several areas. First of all, it is required to study and classify in detail hazardous pests and pathogens, select methods for accounting and identifying in accordance with their degree of danger and the quality category of planting material. Apply comprehensive measures to prevent the entry of pests in the territory of growing planting material and timely eliminate the centers of potential threat.

On the basis of phytosanitary monitoring of actinidia plants in three regions of the Central Federal District, it was found that the greatest leaf damage is caused by the imperfect fungus Alternaria alternata (Fr.) Keissl. The highest prevalence of alternariosis was noted in Actinídia kolomíkta, the maximum degree of pathogen development was noted in the Polish variety Adam (R = 12 %). An increase in the degree of damage by Alternaria was fixed with an increase in wind load and the occurrence of mechanical damage to the leaves. In the course of the experiments, it was determined that A. alternata is able to infect all studied species, and the ability of alternariosis to develop under various climatic conditions and its ability to reproduce various types of fruit, berry, and ornamental crops was noted. The susceptibility to alternariosis of A. kolomíkta, A. argúta, A. polygáma, A. giraldii, hybrids A. argúta and A. purpurea was assessed. Low susceptibility to alternariosis was noted in A. kolomíkta: VIR 1, Izobil’naya, Koroleva Sada, Moma, Soroka; A. giraldii: Juliania; A. arguta: Velikansha, Zolotaya Kosa, Lunnaya, Mikhnevskaya, Velikan; A. polygáma: Abrikosovaya, Zhar-Ptitsa, Zlata, Lesnaya; hybrids A. argúta with A. purpurea: Gibridnaya Kolbasinoy, Souvenir. The only fungal disease on the fruit is gray rot (the causative agent of fungi of the genus Botrytis), the differences in damage between varieties are insignificant, and the degree of development of the disease did not exceed 3 %. Among pests, the polyphage, the common spider mite (Tetranychus tellarius), has the greatest distribution in actinidia plants. Varieties A. argúta, A. polygáma, A. giraldii showed the least susceptibility to phytopathogens and phytophages. In the studied Actinídia species, diseases and pests of the root system are not fixed.

Monilia fruit rot is one of the most common diseases of various fruit and subtropical crops on the Black Sea coast of the Caucasus. Moniliosis of fruit crops is caused by three fungi species of the genus Monilinia — Monilinia fructigena (Pers.) Honey, M. laxa (Aderh. & Ruhland) Honey and M. fructicola (G. Winter) Honey. According to the literature data, among the species of the genus Monilinia, the most common and harmful phytopathogenic species on the Black Sea coast of the Caucasus is M. fructigena. About the presence of the species M. fructicola in the Krasnodar Territory, in particular, and in Russia as a whole, there was no information before the present studies. This pathogen is included both in the EPPO Alert List and in the Unified List of Quarantine Objects of the Eurasian Economic Union. Traditionally, the identification of species of the genus Monilinia occurs by visual method according to cultural-and-morphological characteristics. However, this method does not provide accurate data on the Monilinia species presented in the region. The subject of the experiment was samples of pome fruits and stone fruits (fruits, shoots, buds) with obvious symptoms of moniliosis. Samples were collected at different points in the humid subtropics of the Krasnodar Territory (Russia) and the Republic of Abkhazia. Species identification was carried out using speciesspecific markers. In the process of PCR-analysis for the species-specificity of fungi of the genus Monilinia, a quarantine species M. fructicola was revealed, which was not observed earlier in the territory of the European Economic Union and in the Republic of Abkhazia. M. fructicola was found on peach collected in Sochi and in Abkhazia (Gulripshsky district). To confirm the correctness of the species determination of the discovered fungi of the genus Monilinia, sequencing was performed. The molecular diagnostic method has proved to be promising for the accurate identification of fungi of the genus Monilinia, the causative agent of fruit rot of fruit and subtropical crops.